Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Cryopreservation induces oxidative stress on sperm due to an increase in the number of reactive oxygen species (ROS), thereby resulting decreased quality. ROS's destructive potential is normally counteracted by their innate antioxidant system consisting enzymes, which include superoxide dismutase (SOD). This study aimed assess quality semen from Itik Pinas-Khaki (IP-Khaki) drakes that were cryopreserved with either 4.5% DMSO or 7.0% glycerol as cryoprotectant through evaluation total motility (%) and determination SOD activity (U/mL). Here, samples collected 12 sexually mature IP-Khaki drakes, improved egg-type breed Philippine mallard duck, processed using modified reported cryopreservation procedure for ducks. Results showed post-thawing averages 12.04±5.61% 13.99±5.28% comparable. Moreover, similar levels 0.39±0.18 U/mL 0.33±0.21 2.00 x 108 cells also observed. The observed very low intracellular indicates severe damage cryopreservation, resulted a comparably cryoprotectants. Thus, protocol used not optimum based considerable decline after cryopreservation.